Table 3.

Comparison of three different methods for detection of bacterial virulence factors

Sample no.StrainResults of methods detecting bacterial virulence factors in genea:
rfbEfliCeaeAslt-Islt-IIipaH
HPM1M2HPM1M2HPM1M2HPM1M2HPM1M2HPM1M2
1EC536++++++++++++++++
2EC488++++++++++++++++++++
3S. dysenterii567+++++++++
4S. enterica serovar Enteritidis?+
5ECOR48++?
6DEC5A++++++++?
7DEC5C++++++++
8S. flexneriM90T?++++
9EC503++++++++++++++++++++
10TB285+++++
11KL10035+
12KL10016+?++++
13K-12 W3110+
14TB334+++++
15EC535++++++++++++++++++++
  • a Positive (+), ambiguous (?), and negative (−) results of microbial gene detection are given. H, colony hybridization test; P, PCR product analyzed using agarose gel electrophoresis; M1 and M2, microarray tests, each using one of two independent oligonucleotide targets.