Table 3.

Mean CT values and standard deviations of a dilution series of the nuc gene of S. aureus using the TaqMan probe with the LightCycler or the ABI PRISM 7700 SDS and different reaction mixes

SystemMean CT value ± SD
3 × 100 copies/μl3 × 101copies/μl3 × 102copies/μl3 × 103copies/μl3 × 104 copies/μl
LightCycler with a commercial kit36.72 ± 0.12833.02 ± 0.24029.80 ± 0.10526.46 ± 0.16722.32 ± 0.147
LightCycler with Platinum Taq DNA polymerase34.65 ± 0.21431.11 ± 0.24327.99 ± 0.06424.76 ± 0.06821.29 ± 0.052
ABI PRISM 7700 SDS with Platinum Taq DNA polymerase35.07 ± 0.36731.49 ± 0.06928.56 ± 0.20825.74 ± 0.121NMa
  • a NM, not measured.