Table 2.

Plasmids and oligonucleotides used in this studya

NameDescription or sequence
Plasmids
 pGAP11.9-kb fragment containing GAP1 in pUC19
 pTDH3-AUR1C3.1-kb fragment containing TDH3-AUR1-Cin pUC19
 pΔGAP1gap1::TDH3-AUR1-C in pUC19
 pUC-SFA12.5-kb fragment containing TDH3-SFA1in pUC119
 pUC-SSY11.4-kb fragment containing SSY1 (nucleotide positions −715 to −4 and 1817 to 2550) in pUC18
 pΔSSY1ssy1::TDH3-SFA1 in pUC18
 pYCGPYCentromere-based vector withTDH3-G418r
 pBAP2Sph4.6-kb SphI fragment containing BAP2 in pUC18
 pBAP2ES4.6-kbSphI/Eco47III fragment containing BAP2in pUC18
 pBAP2ORF2.0-kb SacI/KpnI fragment containing BAP2 in pUC18
 pYCGPYBP2BAP2 in pYCGPY
 pYCGPYLgBPLg-BAP2 in pYCGPY
Oligonucleotides
 0015′CTCGAGCTCATGAGTAATACTTCTTCGT-3′
 0025′-CTCGGATCCCTTTAGATTAATGACGAGA-3′
 0035′-TCAGAGCTCGGTACCGGAGCTTACCAGTTCTCA-3′
 0045′-TCAAAGCTTCTGTTTATGTGTGTTTATTCG-3′
 0055′-AAGCTTAATGTCCGCCGCTACTGTTGG-3′
 0065′-GTCGACGTTGGTAGTTAGGAACAGGC-3′
 0075′-GGTACCGGAGCTTACCAGTTCTCACA-3′
 0085′-TAAAAGCTTCTGTTTATGTGTGTTT-3′
 0095′-GAATTCAGGAAAAACTCGAGAGTTACTAG-3′
 0105′-GGTACCTCAAGGAACTTCCCTATTTTTAAGC-3′
 0115′-GGTACCCAGTGTCGACAGGTTATTCTAGTCCTTGGGTTG-3′
 0125′-GGATCCAGGTAACCAACTTCTTCGCTCTT-3′
 0135′-GATCACCCGGGT-3′
 0145′-AAGCTTAGATCTTCAGAACTAAAAAAATAATAAGGAA-3′
 0155′-TCTAGAGCTCTGTGATGATGTTTTATTTGTTTTGATT-3′
 0165′-TCTAGAACAGGATCCTTGGTTGAACACGTTGCCAAG-3′
 0175′-GAATTCACTAGTGATCCGGAGCTTTCAATCAAT-3′
 0185′-GAGCTCTTTGAATGCCATTATCAGAAGAC-3′
 0195′-GCGGCCGCTAACGACTAGTGTCCGAACTTTA-3′
  • a The underlined nucleotides were added to generate restriction sites.