Table 4.

Phylogenetic assignment and theoretical T-RF sizes of 51 16S rRNA gene sequences obtained from the clay fraction of the AM treatment as well as the location in soil of corresponding T-RFs in T-RFLP profiles

Phylogenetic assignmentTheoretical T-RF size (bp)Size of corresponding T-RF in T-RFLP (bp)Location in soil
Holophaga/Acidobacterium 3739All fractions (may also derive from gram-positives andProteobacteria)
76a 76Silt and clay (may also derive from gram-positives)
9292Silt and clay
96a 96Silt and clay
206205All fractions (may also derive fromProteobacteria)
214216Silt and clay
220220Silt and clay
222223Mainly silt and clay (may also derive from Prosthecobacter)
233a 233Clay
234236Silt and clay
235236Silt and clay (may also derive from Prosthecobacter orFlexibacter/Cytophaga/Bacteroides)
242242Clay
Verrucomicrobiales (Prosthecobacter) 100101Silt and clay
208208All fractions
222228Mainly silt and clay (may also derive from Holophaga/ Acidobacterium)
225b
236236Silt and clay (may also derive from Holophaga/Acidobacterium)
237236Silt and clay
Gram-positives
 High G+C3739All fractions (may also derive from Holophaga/Acidobacterium orProteobacteria)
3839All fractions (may also derive from Holophaga/Acidobacterium orProteobacteria)
6362Mainly silt and clay
76a 76Silt and clay (may also derive from Holophaga/Acidobacterium)
132c
210a
229228All fractions
 Low G+C88a
231231All fractions
232231All fractions
240242Clay
284284Clay
290d 290Mainly sand
Proteobacteria
 α3939All fractions (may also derive from Holophaga/Acidobacterium or gram-positives)
6466All fractions
8381All fractions
195194All fractions
 γ3939All fractions (may also derive fromHolophaga/Acidobacterium or gram-positives)
69b 69Silt and clay
 δ211a 212All fractions
 Unclassified206205All fractions (may also derive fromHolophaga/Acidobacterium)
Planctomycetes 230231All fractions
Flexibacter/Cytophaga/ Bacteroides 213a 212All fractions
235236Silt and clay (may also derive from Holophaga/Acidobacterium andProsthecobacter)
  • a Was detected in the T-RFLP profile of the AM clay fraction, but was lost during the normalization procedure.

  • b Was not found in the T-RFLP profile of the AM clay fraction.

  • c Was not detected in any T-RFLP profile.