Table 2.

Enzyme activities in extracts of G. nitidaLE31 grown on 3-EP, 3-MP, and acetate as sole sources of carbon

EnzymeProduct measuredSp act (mUa/mg of protein) for the following growth substrate:
3-EP3-MPAcetate
Isocitrate dehydrogenaseNADH7349
Levulinic aldehyde dehydrogenaseNAD282411
Methylsuccinic aldehyde dehydrogenaseNAD<1<11
2-Methylglutaric aldehyde dehydrogenaseNAD2<1<1
FormamidaseNH3 2419<1
  • a One unit is defined as the amount of enzyme that produces 1 μmol of product per min.