Table 2.

Identities of clones related to numbered bands in Fig. 5, as determined by partial or nearly complete 16S rDNA sequencing

BandAccession no.Closest relative in GenBank database (accession no.)% SimilarityPhylogenetic group
1AY013676aDesulfosporosinussp. strain S10 (AF07527)96Low-G+C-content gram-positive bacteria
2AY013696a, bGallionella ferruginea (L07897 )94β-Proteobacteria
AY013693bUnculturedDuganella sp. strain CTHB-18 (AF067655 )93β-Proteobacteria
3AY013670Unidentified β-proteobacterium cda-1 (Y17060 )96β-Proteobacteria
AY013688Unidentified β-proteobacterium cda-1 (Y17060 )96β-Proteobacteria
AY013694a, bGallionella ferruginea (L07897 )95β-Proteobacteria
AY013698a, bGallionella ferruginea (L07897 )93β-Proteobacteria
AY013691bActinomyces sp. (X92701 )96High-G+C-content gram-positive bacteria
AY013663Uncultured bacterium RB25 (Z95718 )88Unclassified
4AY013697a, bGallionella ferruginea (L07897 )92β-Proteobacteria
AY013695bUnidentified bacterium BD4-9 (AB015559 )88Unclassified
5AY013690aAzoarcus sp. strain BS5–8 (AF011350 )93β-Proteobacteria
6AY013666aAzoarcus sp. strain BS5–8 (AF011350 )93β-Proteobacteria
7AY013669aAzoarcus sp. strain BS5–8 (AF011350 )94β-Proteobacteria
AY013681aAzoarcus sp. strain BS5–8 (AF011350 )94β-Proteobacteria
8AY013674Unidentified bacterium DGGE band 10 (AJ009652 )98High-G+C-content gram-positive bacteria
AY013684aAzoarcus sp. strain BS5–8 (AF011350 )98High-G+C-content gram-positive bacteria
AY013664aDenitrifying bacterium 72Chol (Y09967 )95β-Proteobacteria
AY013675aAzoarcus sp. strain BS5–8 (AF011350 )93β-Proteobacteria
AY013689Uncultured bacterium t0.6.f (AF005745 )91Green nonsulfur bacteria
AY013682Candidate division OP11 clone OPd29 (AF047561 )90Unclassified
9AY013665aDesulfovibrio aminophilus (AF067964 )93δ-Proteobacteria
10AY013593Acetobacterium carbonolicum(X96956 )98Low-G+C-content gram-positive bacteria
AY013613Acetobacterium wieringae(X96955 )97Low-G+C-content gram-positive bacteria
AY013610bAcetobacterium malicum (X96957 )97Low-G+C-content gram-positive bacteria
AY013607bAcetobacterium malicum (X96957 )95Low-G+C-content gram-positive bacteria
11AY013591Acetobacterium wieringae(X96955 )98Low-G+C-content gram-positive bacteria
12AY013609a, bGeobacter akaganeitreducens (U96918 )94δ-Proteobacteria
13AF013603Uncultured eubacterium WCHB1–21 (AF505080 )96Low-G+C-content gram-positive bacteria
14AY013658Uncultured freshwater bacterium (AF109142 )98Unclassified
15AY013644a, bGeobactersp. strain CdA-2 (Y19190 )96δ-Proteobacteria
16AY013634aMetal-contaminated soil clone K20-06 (AF145810 )98δ-Proteobacteria
AY013642aMetal-contaminated soil clone K20-06 (AF145810 )95δ-Proteobacteria
AY013647a, bGeobactersp. strain CdA-2 (Y19190 )96δ-Proteobacteria
AY013648a, bGeobactersp. strain CdA-2 (Y19190 )94δ-Proteobacteria
AY013651aGeobacter sp. strain CdA-3 (Y13131 )93δ-Proteobacteria
AY013641aAzoarcus sp. PCR strain (X85434 )93δ-Proteobacteria
AY013649bUncultured bacterium WCHB1–60 (AF050598 )91Candidate division WS5
17AY013652 /3a, cGeobactersp. strain (GSPY19190)96δ-Proteobacteria
18AY013646bAcidovorax sp. strain UFZ-B517 (AF235010 )96δ-Proteobacteria
AY013643bRhodoferax fermentans (D16211 )96δ-Proteobacteria
AY013650bAcidovorax devluvii (Y18616 )91δ-Proteobacteria
19AY013645a, bGeobactersp. strain CdA-2 (Y19190 )95δ-Proteobacteria
AY013633Eubacterium limosum(M59120 )94Low-G+C-content gram-positive bacteria
AY013638Uncultured bacterium clone H1.4.f (AF005748 )93Green nonsulfur bacteria
20AY013620aUncultured sulfate-reducing bacterium 368 (AJ389629 )91δ-Proteobacteria
21AY013625Uncultured clone CRE-FL35 (AF141457 )97β-Proteobacteria
  • a The identity of the closest relative in the GenBank database gives an indication of the ability to perform redox reactions (microaerophilic, denitrification, iron reduction, or sulfate reduction).

  • b The 16S rDNA was almost completely sequenced.

  • c E. coli positions 8 to 518 and 1002 to 1512 were sequenced.