Table 2.

Comparison of a 1,482-bp sequence of the 16S rDNA genes from different Bacillus isolatesa

IsolateNucleotide at position:No. of differences
60737511616517518026999810021013112914061445
33679ATTGCYGAACGACT
14579ATTGCCGAACGACT1
11778ATTGCCGAACGACT1
31293ATTGCCGAACGACT1
43881ATTGYTGAACGACT2
VollumATTGCCGACCGWCT3
SterneATTGCCGACCGWCT3
AH526ATTGCCGACCGACT2
10792GCAGCTGAACGACT4
53522GCAGCTGAACGAMT5
AH533GCAGYTGAACGACT5
AH540RYWGCTGAACGACA5
AH527GCAGYTGAACGACA6
AH521ATTATTAGATAACA8
AH648ATTATTAGATAACA8
AH665ATTATTAGATAACA8
AH678ATTATTAGATAACA8
  • a A 1,482-bp amplicon containing a bacterial 16S rDNA gene was amplified from 17 differentBacillus isolates, and the amplified DNA fragments were sequenced. Samples analyzed included B. thuringiensis subsp. kurstaki (ATCC 33679),B. cereus ATCC 14579, B. cereusATCC 11778, B. cereus ATCC 31293, B. cereus ATCC 43881, B. anthracis isolates Vollum and Sterne, Norwegian B. thuringiensis isolate AH526, B. thuringiensis ATCC 10792, B. cereus ATCC 53522, six Norwegian B. thuringiensis isolates (AH521, AH533, AH648, AH665, and AH678), and two Norwegian B. cereus isolates (AH527 and AH540). Sequence results were used to generate a phylogenetic analysis (Fig. 6.) A, C, G, and T refer to the four deoxynucleotides that comprise DNA. M refers to an A or C at that position; R refers to a purine (A or G) at that position; Y refers to a pyrimidine (C or T) at that position; and W refers to an A or T at that position. There are multiple copies of the 16S rDNA gene in these bacteria, and some show differences in sequence among their different alleles. Isolates Vollum and Sterne were provided by the U.S. Army Medical Research Institute of Infectious Diseases, Ft. Detrick, Md. “AH” isolates were provided by the Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway.