Table 4.

Genuine → cassette gene conversion is RecA dependent

StrainGenotypeSmr frequency per 10,000 cellsaFold reductionb
R960c rpsL41 cbp3::kan-rpsL+hex + 0.47 ± 0.175.3
R989c R960 butrecA 0.012 ± 0.0057207.5 (39.2d)
R960 rpsL41 cbp3::kan-rpsL + hex + 2.49 ± 0.621.0
R1004R960 but hexA::cat 2.40 ± 0.631.0
R1005R990 buthexA::cat 1.95 ± 0.931.3
  • a Calculated from four to five independent cultures grown from individual colonies resuspended in 2 ml of Todd-Hewitt broth supplemented with 0.5% yeast extract and grown to an OD550 of 0.5 to 0.7 before plating.

  • b Compared to the frequency of spontaneous conversion with the rpsL41-rpsL + combination (hex + background) for cultures grown in the presence of O2 (last three lines; results taken from Table5).

  • c Colonies and cultures grown in the absence of O2.

  • d Fold reduction compared to wild type cultures grown in the absence of O2.