Table 1.

Probes used in the present studya

ProbeProbe sequence (5′→3′)SpecificityFA (%)Source or reference
EUB338GCTGCCTCCCGTAGGAGTBacteria01
ALF968GGTAAGGTTCTGCGCGTTα-Proteobacteria3518
GAM42aGCCTTCCCACATCGTTTγ-Proteobacteria3528
CF319aTGGTCCGTGTCTCAGTACCF cluster3529
COL189AUCCCCCCCUUUGGUCCG Colwelliaclade35This study
COL189h5TAGACRTTATGCGGTATTAGCHelper for COL18935This study
COL189h3CAAATGGCGAGAGGTCCGAAGHelper for COL18935This study
SAR86/1245TTAGCGTCCGTCTGTATSAR86 cluster20This study
SAR86/1245h5CCATTGTAGCACGTGTGTAGCHelper for SAR86/124520This study
SAR86/1245h3GGATTRGCACCACCTCGCGGCHelper for SAR86/124520This study
RSB67CGCTCCACCCGAAGGTAG Roseobacterclade2043
RSB67h5CGCTCGACTTGCATGTGTHelper for RSB672043
RSB67h3CGTTACTAACCCGTCCGCHelper for RSB672043
  • a Abbreviations: FA, formamide; CF,Cytophaga-Flavobacterium.