Table 1.

Oligonucleotide probes developed for FISH screening of environmental samples

ProbeSpecificityProbe sequence (5′-3′)Target sitea (16S rRNA positions)% FAb
CYT1448 Cytophagaspp.CTAGGCCGCTCCTTACGG1448–146530
CYT1438 Cytophagaspp.CCGCTCCTTACGGTGACG1438–145530
NOR5-730NOR5 clusterTCGAGCCAGGAGGCCGCC730–74730
NOR5-130NOR5 isolate KT71CCCCACTACTGGATAGAT130–14720
ROS537Marine alpha cluster (20)CAACGCTAACCCCCTCC537–55335
KT13-231KT1117ATCTAATCAAACGCGGGCC231–24930
KT09bKT0917GCTATTCCGTAGATCTGG136–15320
KT09cKT0917ACTGTGTCCCCTAAAGGA1025–104220
RC1031KT0202aACCTGTCACTATGTCCCG1031–104920
RC1239KT0202aTAACTCACTGTAGTTGCCAT1239–127920
Ros7-1029JP7.1 groupCTGTCACTTGGTCTCTTG1029–104635
Erythro69JP13.1 groupGCCACTCACCCCGAGGGT69–10620
Rhizo218JP66.1 groupGGGCCCATCCTACTCCGA218–23520
  • a E. coli numbering (3).

  • b Percent (vol/vol) formamide (FA) in hybridization buffer.