Table 5.

Mismatch repair-dependent reduction of conversion frequency using the rpsL1-rpsL + allele combination and a hex + genetic background

StrainGenotypeHexMismatchaSmrfrequency per 10,000 cellsbFold reductionc
R960 rpsL41 cbp3::kan-rpsL + hex + +HE2.49 ± 0.621.0
R990d rpsL1 cbp3::kan-rpsL + hex + +LE0.14 ± 0.02018
+LE0.11 ± 0.02723
R1004R960 buthexA::cat HE2.40 ± 0.631.0
R1005R990 buthexA::cat LE1.95 ± 0.931.3
  • a HE, essentially not recognized or corrected by Hex; LE, transition mismatch recognized or corrected out by Hex.

  • b Calculated from four or five independent cultures grown from individual colonies resuspended in 2 ml of broth and grown to an OD550 of 0.5 to 0.7 before plating.

  • c Compared to therpsL41-rpsL + combination (hex + background).

  • d Duplicate experiments on two different occasions are listed for R990.