Table 1.

Similarities of dead aboveground Spartinabiomass NifH amino acid sequences to the most similar sequences from known diazotrophic bacterial speciesa

DeadSpartina sequence(s)Organism(s) with most similar sequence(s)% Similarity
Presumptive α-Proteobacteria
 NIS2-1 Gluconacetobacter diazotrophicus 95.2
 NIS2-2, NIS2-3, NIS4-2, NIS4-4, NIS5-1, NIS5-2, NIS6-1, NIS6-2, NIS7-1, NIS8-1, NIS9-2, NIS10-2, NIS12-3, NIS13-1, NIS13-2, NIW4-1, NIW6-2 Rhodobacter sphaeroides 94.4
 NIS3-1, NIS6-2, NIS7-3 Azospirillum brasilense 94.4
 NIS3-2, NIS9-1, NIS10-3, NIS14-1, NIS15-2, NIS15-4, NIS16-3 Bradyrhizobium japonicum 97.6
 NIS4-1 Azospirillum brasilense, Gluconacetobacter diazotrophicus 92.8
 NIS4-3 Rhodobacter sphaeroides 95.2
 NIS7-2, NIW2-3, NIW4-2, NIW5-1, NIW8-2, NIW8-3, NIW9-4 Rhizobium sp.94.4
 NIS8-2 Rhodobacter sphaeroides 92.8
 NIS8-3, NIW3-1 Rhodobacter sphaeroides 96.0
 NIS10-1, NIS13-3 Rhodobacter sphaeroides 96.8
 NIS10-4 Azospirillum brasilense 88.0
 NIS11-1 Rhodobacter capsulatus, Rhodobacter sphaeroides 97.6
 NIS11-2 Azospirillum brasilense 92.0
 NIS11-3 Rhodobacter sphaeroides 98.4
 NIS12-1 Bradyrhizobium japonicum, Rhizobium leguminosarum biovar phaseoli95.2
 NIS12-2 Rhodobacter sphaeroides 93.6
 NIS13-4 Herbaspirillum seropedicae, Rhizobiumsp.92.0
 NIS14-3, NIS14-4, NIS15-3, NIS16-4 Rhodobacter sphaeroides 97.6
 NIS15-1 Azospirillum brasilense 91.2
 NIS16-2, NIW8-1, NIW9-1 Rhizobium sp.92.8
 NIW2-2, NIW6-3, SIS5-1 Herbaspirillum seropedicae, Rhizobium sp.92.8
 NIW2-4 Azospirillum brasilense, Gluconacetobacter diazotrophicus 96.8
 NIW3-2, NIW6-1 Herbaspirillum seropedicae, Rhizobium sp.92.0
 NIW3-3, NIW4-3, NIW5-2, NIW6-3, NIW7-1, NIW9-2, NIW9-3 Rhizobium sp.93.6
 NIW4-4 Azospirillum brasilense 98.4
 NIW5-3 Azospirillum brasilense, Gluconacetobacter diazotrophicus 91.2
 SIS1-1, SIS4-4 Azospirillum brasilense, Gluconacetobacter diazotrophicus 94.4
 SIS2-1, SIS4-2 Azospirillum brasilense 100.0
 SIS2-2, SIW1-2 Gluconacetobacter diazotrophicus 94.4
 SIS2-3 Gluconacetobacter diazotrophicus 92.8
 SIS2-5 Azospirillum brasilense 92.8
 SIS2-6 Gluconacetobacter diazotrophicus 86.4
 SIS3-1 Azospirillum brasilense 95.2
 SIS3-2, SIW1-7 Gluconacetobacter diazotrophicus 97.6
 SIS3-3, SIS3-6 Azospirillum brasilense 93.6
 SIS3-4 Azospirillum brasilense, Bradyrhizobium japonicum, Gluconacetobacter diazotrophicus, Rhodobacter sphaeroides 93.6
 SIS3-5 Gluconacetobacter diazotrophicus, Rhizobium sp.83.2
 SIS3-7 Azospirillum brasilense, Gluconacetobacter diazotrophicus 92.0
 SIS3-8, SIW2-3, SIW2-5 Gluconacetobacter diazotrophicus 93.6
 SIS4-1, SIW2-6 Herbaspirillum seropedicae, Rhizobium sp.93.6
 SIS4-3 Azospirillum brasilense 99.2
 SIW1-5 Azospirillum brasilense, Bradyrhizobium japonicum, Gluconacetobacter diazotrophicus, Rhizobium leguminosarum biovar phaseoli94.4
 SIW1-6 Azospirillum brasilense, Gluconacetobacter diazotrophicus 93.6
 SIW2-1 Rhodobacter sphaeroides 96.0
 SIW2-2 Azospirillum brasilense 96.0
 SIW2-4, SIW4-2 Herbaspirillum seropedicae 92.8
 SIW3-1 Gluconacetobacter diazotrophicus 96.0
 SIW4-1 Gluconacetobacter diazotrophicus 91.2
 SIW4-3 Rhizobiumsp.89.6
 SW4-4 Azospirillum brasilense 99.2
 SW4-5 Azospirillum brasilense 97.6
Presumptive γ-Proteobacteria
 NIS1-2 Azomonas agilis, Azotobacter chroococcum 95.2
 NIS1-3 Azomonas agilis, Azotobacter chroococcum 99.2
 NIS1-4 Pseudomonas stutzeri 98.2
 NIS11-4 Azotobacter chroococcum, Azotobacter vinelandii 84.9
 SIS2-4 Vibrio diazotrophicus 86.1
 SIS2-7 Vibrio diazotrophicus 84.2
 SIW1-4 Azomonas agilis 96.8
Presumptive anaerobes
 NIS1-1 Desulfovibrio salexigens 84.2
 NIS3-3 Desulfonema limicola 84.8
 NIS14-2 Desulfonema limicola 82.8
 NIS16-1 Desulfovibrio salexigens 88.1
 SIW1-1 Desulfovibrio salexigens 85.1
  • a Percent similarities are from the distance matrix constructed in MEGA version 2.0. Spartinasequences are listed by the DGGE gel bands from which they were recovered (see Materials and Methods) and grouped into the major sequence clusters shown in Fig. 2 to 4. Note that these clusters are named for the predominant types of organisms that they contain, but some sequences from other phylogenetic groupings can occur.