Table 2.

Comparison of wild-rice-derived diazotrophic bacteria B65, B501, and B512 with nitrogen-fixing Herbaspirillumspp.a

CharacteristicDiazotrophic bacteria from wild riceH. seropedicaeH. rubrisubalbicansH. frisingense
B65B501B512
Basic property
 Gram staining
 Motility++++++
 Cell shapeVibrioidVibrioidVibrioidVibrioidSlightly curved rodSlightly curved rod
 Cell width (μm)0.5–0.60.5–0.60.5–0.60.6–0.70.6–0.70.5–0.7
 Catalase++++++
 Oxidase++++++
Utilization of carbon source
meso-Erythritol++++
N-Acetylglucosamine++
l-Rhamnose++++
meso-Inositol++
 Sebacate+ND
 Adipate++
 Glucose+++ND+
 SucroseND
 Ketoglutarate+++++ND
 Citrate+++NDND
% Similarityb
 HERB 86 (Herbaspirillum-specific)100100100100100100
 HERB 1432 (Herbaspirillum-specific)100100100100100100
 Hsero 445 (H. seropedicae-specific)8394941008888
 Hrubri 445 (H. rubrisubalbicans-specific)9483838310077
 Hfris 445 (H. frisingense-specific)7794948877100
  • a Phenotypic traits data for H. seropedicae, H. rubribalbicans, and H. frisingense are taken from references 11, 24, and 27. ND, not determined.

  • b Percent similarity to diagnostic oligonucleotide sequences of 16S rRNA gene. Complementary 18-nucleotide sequences of genus-and species-specific diagnostic oligonucleotide probes of 16S rRNA (27) (HERB 86, 5′-GCAGCATAGGAGCTTGCT; HERB 1432, 5′-AAGTGGGTAGCCTAACCC; Hsero 445, 5′-GGAAGAAACGGTTTTGGC; Hrubri 445, 5′-GGAAGAAACGGTGGTAGC; Hfris 445, 5′-GGAAGAAACGGTTCTGGA) were compared with 16S rDNA sequences of strains B65, B501, and B512.