Table 2.

Excisional transformation replaces kan-rpsLcassette with cbp3a

Donor DNAInduction by CSPFold increaseb in SmrGrowth (CFU/ml) with selection on:
SmSm and Kn
None+1.11,000830
1910820
R800 (Sms)+2822,0001,000
1780710
  • a Strain CP1296 was grown to an optical density of 0.05, diluted 10-fold, and treated as indicated with CSP-1 (100 ng/ml) and DNA (10 ng/ml) for 60 min. Cell types are reported among 10 to 20 million cells per ml in the transformed population.

  • b Ratio of Smr in CSP-treated culture to that in untreated control.