Table 1.

Environmental sites sampled for gene cassettes

LocationaDescriptionSample typeNo. of samplesbNo. PCR positivecNo. of clones sequenced
Balmain, NSWAbandoned industrial siteContaminated soil6432
Homebush, NSWAbandoned industrial siteContaminated estuarine sediment10520
Macquarie, NSWUrban eucalypt forestSoil83NDd
Lidsdale, NSWMixed plantation forestSoil22ND
Sturt National Park, NSWSemidesertSandy and stony soils954
Nullarbor, SAAquatic caveMicrobial biofilm62ND
Shelley Beach, NSWOcean beachSeawater10ND
Hunter River, NSWShallow riverSediment20ND
Namoi River, NSWShallow riverSediment66ND
Yerranderie, NSWAbandoned silver mineSoil and sediment, low pH292826
Cape Denison, AntarcticaPenguin colonyOrnithogenic soil12108
Flinders Ranges, SAHot spring, 48–63°CMicrobial biofilm and sediment997
Lomatia Creek, NSWUrban creekMicrobial biofilm and sediment666
Jubilee 1, NSWAbandoned dump siteContaminated soil335
Jubilee 2, NSWUrban creekSediment116
  • a Samples used for cloning are represented by the following acronyms: Balmain, Bal; Homebush, HB; Pulgamurtie landform, Sturt National Park, Pu; Yerranderie silver mine, SM; Cape Denison ornithogenic soil, Orn; Flinders ranges hot spring, FR; Lomatia creek sediment, Lom; Jubilee dump, Dum; Jubilee creek sediment, Sed. NSW, New South Wales; SA, South Australia.

  • b Number of independent samples collected and tested with the cassette PCR.

  • c Number of samples generating PCR products. PCR profiles from the same sample were highly reproducible; independent samples collected at the same location showed high levels of intersample variability.

  • d ND, not determined. Libraries were not constructed from these samples.