Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Source or reference
Bacterial strains
Pseudomonas putida IsoFWild-type isolate from tomato rootsThis work
Pseudomonas putidaF117AHL-negative derivative of IsoF; PpuIThis work
Pseudomonas putida KS35F117 with integrated PlasB-gfp(ASV) Plac-lasRcassetteThis work
Pseudomonas putidaZ2DWild-type isolate from tomato rootsThis work
Serratia liquefaciens MG44AHL-negative mutant ofS. liquefaciens MG1; SwrI12
Rahnella aquatilis T13Wild-type isolate from tomato rootsThis work
Rahnella aquatilisTAAWild-type isolate from tomato rootsThis work
Escherichia coli MT102araD139(ara-leu)7697 Δlac thi hsdRLaboratory collection
Escherichia coliHB101recA thi pro leu hsdRM+ Smr24
Escherichia coli CC118 (λpir)Δ(ara-leu) araDΔlacX74 galE galK phoA20 thi-I rpsE rpoB argE(Am)recA1 λpir lysogen20
Plasmids
 pAS-C8pBBR1MCS-5 carrying Pcepl-gfp(ASV)-Plac-cepR; Gmr38
 pKR-C12pBBR1MCS-5 carrying PlasB-gfp(ASV)-Plac-lasR; Gmr38
 pTn5-LASpTn5-based delivery plasmid carrying PlasB-gfp(ASV) Plac-lasR; GmrAprHentzer et al., submitted
 pJBA132pME6031 carryingluxR-PluxR-Plux1-gfp(ASV)-T0-T1; Tetr1
 pSB403Bioluminescent broad-host-range AHL sensor plasmid; Tetr50
 pUT-Kan-dsredpUT-Kan carrying Plac-rfp-T0-T1; Kanr Apr45
 pTn5-RedTn5-based delivery plasmid carrying Plac-dsred-T0-T1; Tetr AprHentzer et al., submitted
 pGEM-3Zf(+)lacZα cloning vector; AprPromega, Madison, Wis.
 pBBR1MCS-5Broad-host-range plasmid; lacZα; Gmr25
 pRK600ColE1 RK2-Mob+RK2-Tra+; helper plasmid; Cmr8