Table 1.

Microbial strains and plasmids used in this study

Strain or plasmidRelevant feature(s)Source or reference
Strains
E. coli
  JM109F′traD36 proA + proB + lacI q Δ(lacZ)M15 Δ(lac-proAB) supE44 hsdR17 recA1 gyrA96 thi-1 endA1 relA1 e14λ 40
  DH5αF φ80dlacZ ΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 hsdR17 (rK + mK )phoA supE44 λ thi-1 gyrA96 relA1 Life Technologies, Rockville, Md.
S. cerevisiae
  CEN.PK2-1C MAT a leu2-3,112 his3-Δ1 ura3-52 trp1-289 MAL2-8(Con) MAL3 SUC3 10
  YUSM1006aCEN.PK2-1C(pUSM1006)This work
  YUSM1009aCEN.PK2-1C (Δgre3)This work
  TMB3101CEN.PK2-1C (xylA LEU2)This work
  TMB3102YUSM1009a (Δgre3 xylA LEU2)This work
  TMB3103CEN.PK2-1C (xylA LEU2 XKS1 TRP1)This work
  TMB3104YUSM1009a (Δgre3 xylA LEU2 XKS1 TRP1)This work
Plasmids
 pBR322 bla tet 3
 pGEM-T easy vector bla Promega Corporation, Madison, Wis.
 pBluescript SK(−) bla GenBank (accession no. 52330)
 pBXI bla PGKp:xylA:PGKt LEU2 37
 pXks bla PGKp:XKS1:PGKt TRP1 9
 pUSM1002 bla GRE3t in pBR322This work
 pUSM1003 GRE3p in pGEM-T easyThis work
 pUSM1004 bla GRE3p This work
 pUSM1006 bla URA3 GRE3p GRE3t This work
 pUSM1007pUC8/URA3 This work
 pUSM1008 bla GRE3t in pBluescript SK(−)This work