TABLE 2.

Growth characteristics of the representative HTCC isolates in the OMG group

CladeStrainSpecific growth rate at 16°C (day−1)Maximum cell density at 16°C (106 cells ml−1)Growth on agar mediaa
LNHMLNHM + 1× MCLNHMLNHM + 1× MC1/10 R2AR2AMA 2216ASWSASWZDSMZ246
OM60HTCC21481.572.191.053.03GGG
HTCC22462.092.110.890.87G
HTCC20802.212.221.295.71G
BD1-7HTCC21432.112.320.272.25GGGG
KI89AHTCC20891.562.010.681.47
OM182HTCC21511.351.751.284.00
HTCC21801.231.570.833.49
HTCC21781.941.650.641.20GGGGG
HTCC21882.631.831.325.00GG
SAR92HTCC21211.181.611.269.93
HTCC22901.361.650.733.99
HTCC22072.052.271.3415.7G
  • a Growth on agar media was determined at 16°C. The inocula were the HTCC cultures adapted to laboratory conditions after successive purifications. The original HTCC extinction cultures obtained from natural seawaters did not form colonies on LNHM plus 1 × MC and 1/10 R2A agar media. ASWS, artificial seawater from Schut et al. (47); ASWZ, 1/10 R2A agar in artificial seawater from Zobell's formula 2 (50); DSMZ246, 1/10 R2A agar in artificial seawater from DSMZ medium 246; G, growth; −, no growth.