Source | RFLP analysis results | Nucleotide sequence analysis results | |||||
---|---|---|---|---|---|---|---|

S^{a} | Estimated S_{e}^{b} | H^{c} | E (H/H_{max})^{d} | C (%)^{e} | Avg sequence similarity (%)^{f} | D_{mean} (substitutions/100 sites)^{g} | |

Native | 86 (210) | 264 ± 74 | 4.87 | 0.76 | 59 | 85.9 ± 0.7 (100)^{h} | 16.98 ± 13 |

Cultivated | 104 (146) | 301 ± 78 | 6.47 | 0.97 | 28 | 74.6 ± 0.7 (100)^{h} | 26.31 ± 18 |

↵

*a*Phylotype richness,*S,*was calculated as the total number of unique RFLP patterns in soil. Numbers in parentheses indicate the overall numbers of clones of the expected size screened by RFLP.↵

*b*Estimated phylotype richness,*S*, as estimated by nonparametric Chao1 estimator (9). The data are the mean ± standard error of the mean._{e}↵

*c*Shannon diversity index (22).↵

*d*Evenness (22) of population.↵

*e*Library coverage (14).↵

*f*Average sequence similarity (± standard error of the mean) from pairwise comparisons of gene fragments.↵

*g*The mean taxonomic distance (± standard error of the mean) from pairwise comparisons of gene fragments (32, 48).↵

*h*Sequences with <90% identity to any published sequence recorded in the NCBI database.