TABLE 1.

Summary of data from RFLP and nucleotide analyses of nosZ gene fragments from denitrifier communities in native and cultivated soils

SourceRFLP analysis resultsNucleotide sequence analysis results
SaEstimated SebHcE (H/Hmax)dC (%)eAvg sequence similarity (%)fDmean (substitutions/100 sites)g
Native86 (210)264 ± 744.870.765985.9 ± 0.7 (100)h16.98 ± 13
Cultivated104 (146)301 ± 786.470.972874.6 ± 0.7 (100)h26.31 ± 18
  • a Phylotype richness, S, was calculated as the total number of unique RFLP patterns in soil. Numbers in parentheses indicate the overall numbers of clones of the expected size screened by RFLP.

  • b Estimated phylotype richness, Se, as estimated by nonparametric Chao1 estimator (9). The data are the mean ± standard error of the mean.

  • c Shannon diversity index (22).

  • d Evenness (22) of population.

  • e Library coverage (14).

  • f Average sequence similarity (± standard error of the mean) from pairwise comparisons of gene fragments.

  • g The mean taxonomic distance (± standard error of the mean) from pairwise comparisons of gene fragments (32, 48).

  • h Sequences with <90% identity to any published sequence recorded in the NCBI database.