TABLE 3.

Plasmids used

PlasmidRelevant propertiesSource or reference(s)
pBluescript II SK+Standard cloning vectorStratagene
pD4pBluescript KS with H. volcanii 3,566-bp MboI-HindIII fragment containing hdrB gene26
pGB68pBR322 with NovR and flanking sequences of pyrE23
pGB70pUC19 with pyrE2 under ferredoxin promoter3
pMDS99Shuttle vector based on pOK12 with pHV2 replication origin and MevR from Haloarcula hispanica31
pWL102Shuttle vector based on pAT153 with pHV2 replication origin and MevR from H. volcanii7, 22
pTA44pBluescript II with H. volcanii 4,162-bp BssHII fragment containing leuB geneThis study
pTA49pBluescript II with H. volcanii 3,676-bp Sau3AI fragment containing trpA geneThis study
pTA65pBluescript II with BamHI PCR fragment containing flanking regions of leuBThis study
pTA70pTA65 with NotI fragment from pMDS99 containing MevRThis study
pTA73pGB70 with XbaI-HindIII ΔleuB fragment from pTA65This study
pTA92pBluescript II with XbaI PCR fragment containing flanking regions of trpAThis study
pTA93pTA92 with NotI fragment from pMDS99 containing MevRThis study
pTA95pGB70 with XbaI ΔtrpA fragment from pTA92This study
pTA131pBluescript II with BamHI-XbaI fragment from pGB70 containing pyrE2 under ferredoxin promoterThis study
pTA132pBluescript II with PCR fragment containing trpA under ferredoxin promoterThis study
pTA133pBluescript II with PCR fragment containing leuB under ferredoxin promoterThis study
pTA150pBluescript II with H. volcanii 3,974-bp XhoI-NruI fragment containing lhr geneThis study
pTA155pTA131 with HindIII-XbaI PCR fragment containing flanking regions of hdrBThis study
pTA166pTA131 with XhoI-SpeI PCR fragment containing flanking regions of lhrThis study
pTA172pTA166 with PCR fragment containing trpA under ferredoxin promoter, inserted at site of lhr deletionThis study
pTA192pBluescript II with PCR fragment containing hdrB under ferredoxin promoterThis study
pTA230pTA131 with NcoI-HindIII fragment from pWL102 containing pHV2 replication originThis study
pTA231pTA132 with NcoI-HindIII fragment from pWL102 containing pHV2 replication originThis study
pTA232pTA133 with NcoI-HindIII fragment from pWL102 containing pHV2 replication originThis study
pTA233pTA192 with NcoI-HindIII fragment from pWL102 containing pHV2 replication originThis study