TABLE 1.

Summary of clone libraries and phylogenetic groups detected

Source of DNALibrary namePrimerPCR annealing temp (°C)Target sequences detectedc
Marine sediment (California)BMSbOP11-1090R/27F52Y
Marine sediment (California)BoIbOP11-1090R/27F55Y
Marine microbial mat (California)BTMbOP11-1090R/27F52Y
Landscaped topsoil (California)DAOP11-1090R/27F52Y
Landscaped topsoil (California)DB11_1_502F/1525R60Y
Sulphur river (Kentucky)SRA11_3_1368R/27F60Y
Sulphur river (Kentucky)SRB11_5_1380R/27F60Y
Sulphur river (Kentucky)SRCOP11-1090R/27F52Y
Sulphur river (Kentucky)SRD11_2_1236R/27F56Y
Contaminated aquifer (Michigan)WSA11_2_1236R/27F56Y
Marine sediment (California)BMSA11_3_1368R/27F60N
Marine sediment (California)BMSB11_1_502F/1525R60N
Marine sediment (California)BolA11_3_1368R/27F60N
Marine sediment (California)BolB11_1_502F/1525R60N
Fairfax swamp (Indiana)FSA11_3_1368R/27F60N
Oxidized iron deposits (Colorado)GalA11_1_502F/1525R60N
Gallionella Mat (Colorado)GalB11_3_1368R/27F60N
Lake Lemon sediments (Indiana)LLB11_3_1368R/27F60N
Pool N10, Norris Geyser Basin (YNP)aN10A11_1_502F/1525R60N
Green Finger Pool, (YNP)O1aA11_3_1368R/27F60N
Green Finger Pool, (YNP)O1aBOP11-1090R/27F52N
Obsidian Pool (YNP)OBPB11_3_1368R/27F60N
Pavillion Lake (British Columbia, Canada)PLTA11_3_1368R/27F60N
Quenn's Laundry (YNP)QL2A11_3_1368R/27F60N
Quenn's Laundry (YNP)QL2BOP11-1090R/27F52N
Sulphur river (Kentucky)SRE11_1_502F/1525R60N
Contaminated aquifer (Michigan)WSB11_1_502F/1525R60N
  • a YNP, Yellowstone National Park.

  • b Hemi-nested PCR.

  • c Y, yes; N, no.