TABLE 3.

Summary of features of presumed protein contamination of the MM of M. gryphiswaldense identified by various sequencing methods

Protein1D band no.2D spot no.No. of peptidesBest Blast hit (accession no.)aE value
2D PAGE + MS-MSTotal tryptic digestion + MS-MS
Outer membrane protein7, 84 Bracella melitensis (Q44662 )4e-54
ATP synthase alpha8, 96 Rhodospirillum rubrum (PO5036 )3e-11
ATP synthase beta6661 Rhodospirillum rubrum (PO5038 )0.0
Ribosomal protein S144 Bradyrhizobium japonicum (NP_767380 )0.0
EF-Tu742 Masorhizobium loti BAC50667 (NP_102118 )1e-07
FusA15 Rickettsia bellii (AAM90927 )0.0
DnaK36 Rhodopseudomonas sp. (O05700 )0.0
GroEL510 Rhizobium meliloti (NP_437546 )0.0
Cytochrome c11 Rhodospirillum rubrum (P23135 )1e-63
cb-type cytochrome c oxidase CcoO subunit1 Brucella melitensis (NP_540482 )1e-90
Cytochrome b2 Bradyrhizobium japonicum (NP_769126 )1e-140
  • a Excluding hits to other MTB.