TABLE 4.

Growth characteristics of selected H. volcanii mutants

StrainGenotypeGrowth on:
Hv-YPCHv-CaHv-Min
H26ΔpyrE2+UraUra
H37ΔleuB++Leu
H77ΔtrpA+TrpTrp
H66ΔpyrE2 ΔleuB+UraUra Leu
H53ΔpyrE2 ΔtrpA+Ura TrpUra Trp
H98aΔpyrE2 ΔhdrBThyUra ThyUra Thy
  • a In addition to thymidine, ΔhdrB strain cultures should be supplemented with hypoxanthine in Hv- Ca and with hypoxanthine, methionine, glycine, and pantothenic acid in Hv-Min (26).