TABLE 3.

Statistical ANOVA of different treatments tested in detail for phytoplankton viruses and heterotrophic bacteriophages together (6 to 10 viruses tested per treatment)a

ExptFactor treatmentLevelsLog
CountsGFLSSC
IncubationTemp5**
IncubationTime7*
FixationGlutaraldehyde4
FixationFormaldehyde4*
SYBR Green 1Dye concn6*
SYBR dyesGold vs Green I2 (yes/no)*
SYBR GoldDye concn3*
DilutionSolution5*
TE solutionpH6
Dilution factorAddition of seawater7**
AdditivesDetergents5**
AdditivesCitrate7
StorageT = 1 h4*
StorageT = 1 mo4****
Storage4°C4****
  • a A two-way ANOVA with viruses as a random factor (block factor) and a treatment on two to seven levels was used. The treatments that were found to significantly affect total viral counts, GFL, or SSC of the stained virus particles are indicated by asterisks: *, P < 0.05; **, P < 0.01; and ***, P < 0.001. Levels are as described in Materials and Methods.