TABLE 2.

Cellular fatty acid profiles and crude protein contents of E. coli O157:H7 grown at 30°C in TSB for 22 h and in TSB supplemented with 5.0% ethanol for 30 h

Fatty acid or proteinE. coli O157:H7 straina
SEA 13B8830-2C4
Without ethanolWith 5.0% ethanolWithout ethanolWith 5.0% ethanol
Fatty acid (mg/g of freeze-dried cells)
    Dodecanoate (n-12:0)1.35 ± 0.102.22 ± 0.141.51 ± 0.092.54 ± 0.05
    Tetradecanoate (n-14:0)4.28 ± 0.184.66 ± 0.514.95 ± 0.455.15 ± 0.57
    3-Hydroxytetradecanoate (3-OH 14:0)2.50 ± 0.413.88 ± 0.622.99 ± 0.244.05 ± 0.16
    cis-9-Hexadecenoate (cis-16:1, 9)0.29 ± 02.13 ± 0.320.33 ± 0.021.68 ± 0.64
    Hexadecanoate (n-16:0)11.77 ± 0.3715.00 ± 2.4613.05 ± 0.9815.61 ± 0.79
    Heptadecanoate (n-17:0)8.14 ± 0.219.02 ± 1.568.51 ± 0.679.28 ± 0.68
    cis-9-Octadecenoate (cis-18:1, 9)0.32 ± 0.04ND0.36 ± 0.06ND
    trans-9-Octadecanoate (trans-18:1, 9)0.40 ± 0.025.54 ± 1.100.50 ± 0.024.55 ± 0.22
    Nonadecanoate (n-19:0)5.90 ± 0.324.50 ± 0.796.20 ± 0.414.50 ± 1.00
    Total34.95 (3.50%)46.95 (4.70%)38.40 (3.84%)47.36 (4.74%)
Crude protein content (% [wt/wt] N × 6.25, freeze-dried cells)82.81 ± 1.3286.94 ± 0.9388.44 ± 1.0290.62 ± 1.22
Protein content in extracted cell lysates (mg/ml)4.04 ± 0.166.82 ± 0.324.05 ± 0.155.45 ± 0.45
  • a Values are means of two determinations ± the range. ND, none detected.