Screening for oligotrophic bacterioplankton cultures

SampleTotal no. of wells inoculatedNo. of cultures detected in screening IScreening II
No. of cultures showing growth in rich mediumNo. of oligotrophic bacterioplankton culture candidatesNo. of mixed culturesaNo. of cultures lost during transferbNo. of cultures not identifiedNo. of sequenced oligotrophic cultures
Baltic Sea192224184905
Skagerrak Sea384779683431111
  • a Based on mixed sequences.

  • b Cultures did not grow after transfer, or DNA could not be extracted.

  • c Sequenced isolates minus duplicates are shown in Table 2.