TABLE 2.

Purification of the nitrite-eliminating enzyme from N. simplex FJ2-IAa

Purification stepVol (ml)Total protein (mg)Sp act (U/mg)Total activity (U)Recovery (%)Purification (severalfold)
Crude extract35.0340.01.3432.7100.01.0
Q Sepharose9.037.04.1151.035.03.2
Phenyl Superose1.01.640.465.015.031.8
Gel filtration0.40.257.014.33.344.9
  • a Cells were harvested in the exponential-growth phase. The wet-cell mass was 12 g.