TABLE 3.

Purification of the hydrolase from N. simplex FJ2-IAa

Purification stepVol (ml)Total protein (mg)Sp act (U/mg)Total activity (U)Recovery (%)Purification (severalfold)
Crude extract35.0340.20.4129.3100.01.0
Q Sepharose9.065.01.9125.096.05.1
Phenyl Superose3.04.39.528.522.025.0
Gel filtration0.80.524.412.29.064.1
  • a The hydrolase was purified from the same cell extract as the nitrite-eliminating enzyme (see Table 2).