TABLE 1.

Bacterial strains or plasmids

Bacterial strain or plasmidCharacteristicsReference or source
Strains
    Staphylococcus epidermidis strain 5Pep5+ Imm+, wild-type producer of Pep5, harbors pED503 (20 kb)28
    Staphylococcus epidermidis strain 25Pep5 Imm, S. epidermidis 5, pED503 removed9
    Staphylococcus carnosus TM300Cloning host, sensitive to Pep530
    Escherichia coli TB1araΔ(lac-proAB), rpsL(φ80, lacZΔM15), hsdR14
Plasmids
    pCU1Ampr Cmr; 4.95-kb shuttle vector, contains pC194 and pUC19 sequences and multiple cloning site from pUC194
    pAG1/1Ampr Cmr, contains a mutated pepI gene in which Ile17 has been exchanged for Arg cloned in pCU120
    pAG4/1Ampr Cmr, contains a mutated pepI gene in which Phe13 and Ile17 have been exchanged for Asp and Arg, respectively, cloned in pCU120
    pAG4/2Ampr Cmr, contains a mutated pepI gene in which Phe13 and Ile17, and Lys65 have been exchanged for Asp, Arg and Stop, respectively, cloned in pCU1This study
    pTX15Tetr, 7.2-kb staphylococcal expression vector22
    pUP10Tetr, contains 0.244-kb PCR-pepI and 0.024-kb six-His tag cloned in pTX15 (Δlip)This study
    pAH3Tetr, contains 0.772-kb PCR-eciO and 0.024-kb His tag cloned in pTX15 (Δlip)This study
    pEGFP-1Kanr, Neor, 4.2-kb promotorless expression vector contains a 0.720-kb gfp geneBD Biosciences
    pAH-PepI1-68Tetr, contains 0.244-kb PCR-pepI and 0.732-kb PCR-gfp cloned in pTX15 (Δlip)This study
    pAH-EciOTetr, contains 0.772-kb PCR-eciO and 0.732-kb PCR-gfp cloned in pTX15 (Δlip)This study