TABLE 5.

Pep5 susceptibility of strains producing PepI and mutant PepI-Gfp

Plasmid and characteristicMIC (nM) for expression host
S. epidermidis strain 25S. carnosus TM300
No plasmid30.1
pAH-PepI1-681031.5
N-terminal mutations in PepI
    pTS(I17R)-PepI1-6890.3
    pTS(F13D;I17R)-PepI1-68150.35
C-terminal shortening of PepI
    pTS-PepI1-631031.5
    pTS-PepI1-57200.3
    pTS-PepI1-537.50.15
N- and C-terminal mutations in PepI
    pTS(I17R;K59T)-PepI1-6890.3
    pTS(F13D;I17R)-PepI1-63150.35