Specificity of the primers and the primer-probe combination used in this study, tested by qualitative PCR and quantitative real-time PCR, respectively

Qualitative PCRQuantitative real-time PCR
Bifidobacterium adolescentis JCM 1275T++
Bifidobacterium angulatum JCM 7096T++
Bifidobacterium bifidum JCM 1254T++
Bifidobacterium breve JCM 1192T++
Bifidobacterium catenulatum JCM 7130++
Bifidobacterium denticolens DSM 10105T++
Bifidobacterium dentium DSM 20436T++
Bifidobacterium infantis DSM 20088T++
Bifidobacterium longum JCM 1217T++
Bifidobacterium pseudocatenulatum JCM 1200T++
Bacteroides vulgatus DSM 1447T
Clostridium butyricum DSM 10702T
Clostridium coccoides DSM 935T
Enterobacter aerogenes DSM 30053T
Enterococcus faecalis DSM 20478T
Enterococcus faecium Gaio
Escherichia coli K-12
Eubacterium cylindroides DSM 3983T
Eubacterium hallii DSM 3353T
Lactobacillus acidophilus La-5
Lactobacillus gasseri DSM 20077
Lactobacillus jensenii DSM 20557T
Lactobacillus casei DSM 20011T
Lactobacillus paracasei DSM 20244
Lactobacillus plantarum 299v
Lactobacillus rhamnosus GG
Peptostreptococcus anaerobius DSM 2949T
Anaerococcus prevotii DSM 20548T
Ruminococcus hansenii DSM 20583T
Streptococcus thermophilus DSM 20617T
Veillonella dispar DSM 20735T
  • a JCM, Japan Collection of Microorganisms, Saitama, Japan; DSM, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany; T, type strain.