TABLE 1.

Comparison of total 16S rRNA contents from seawater samples and enrichmentsa

SampleDepth (m) or source of enrichment         (sample)        Total 16S rRNA content         (ng/ml)        Total 16S rRNA (no. of molecules/ml)bT. denitrificans-like-organism-specific 16S rRNA fractionTotal cells     (106/ml)c    T. denitrificans- like-organism- specific 16S rRNA gene fraction (%     relative abundance)    No. of T. denitrificans-like cells/mlNo. of ribosomes/ T. denitrificans- like cell
% Relative abundanceNo. of molecules/ml
G151.41 (±0.04)1.62 × 109 (±4.48 × 107)0.003 (±0.003)4.86 × 104 (±1.34 × 103)2.150.012 (±0.002)2.58 × 102 (±4.30 × 101)200
G2300.06 (±0.02)6.59 × 107 (±2.09 × 107)0.059 (±0.018)3.89 × 104 (±1.23 × 104)1.31
G3500.31 (±0.01)3.54 × 108 (±1.46 × 107)0.002 (±0.001)7.10 × 103 (±2.92 × 102)0.53
G4800.21 (±0.00)2.44 × 108 (±9.51 × 105)0.0036 (±0.0024)8.78 × 103 (±3.42 × 101)0.430.021 (±0.007)9.00 × 101 (±3.01 × 101)100
G61200.29 (±0.01)3.32 × 108 (±6.43 × 106)0.083 (±0.038)2.76 × 105 (±5.34 × 104)0.271.552 (±0.158)4.19 × 103 (±4.27 × 102)70
G71380.46 (±0.01)5.24 × 108 (±8.75 × 106)0.916 (±0.251)4.80 × 106 (±8.02 × 104)0.858.346 (±3.110)7.08 × 104 (±2.64 × 104)70
G91750.48 (±0.02)5.53 × 108 (±1.97 × 107)0.466 (±0.075)2.58 × 106 (±9.18 × 104)0.9015.055 (±3.519)1.35 × 105 (±3.17 × 104)20
G102250.04 (±0.00)4.65 × 107 (±3.78 × 106)1.10
Bx21G73.27 (±0.22)3.76 × 109 (±2.63 × 108)8.445 (±0.826)3.18 × 108 (±3.11 × 107)1.6617.850 (±2.709)2.96 × 105 (±4.50 × 104)1,100d
Bx23G93.23 (±0.13)3.71 × 109 (±1.45 × 108)19.099 (±1.070)7.09 × 108 (±3.97 × 107)1.3138.894 (±9.100)5.10 × 105 (±1.19 × 105)1,400e
Bx24G97.74 (±0.10)8.90 × 109 (±1.19 × 107)49.723 (±9.951)4.43 × 109 (±8.86 × 108)4.4349.654 (±8.001)2.20 × 106 (±3.54 × 105)2,000f
  • a Total 16S RNA contents from seawater samples and enrichments with T. denitrificans-like-organism-specific relative and cellular 16S rRNA and 16S rRNA gene abundances and estimated number of ribosomes per T. denitrificans-like cell. Standard deviations are given in parentheses. —, below detection limit.

  • b Based on 1.15 × 109 molecules of 16S rRNA/ng.

  • c Data from Weinbauer et al. (44).

  • d Value indicates a 16-fold increase of ribosome content per cell in comparison to that of the original environmental sample.

  • e Value indicates a 70-fold increase of ribosome content per cell in comparison to that of the original environmental sample.

  • f Value indicates a 100-fold increase of ribosome content per cell in comparison to that of the original environmental sample.