TABLE 1.

PCR primers used in this study

PrimerPositionsaSequence (5′→3′)Annealing tempbReference or source
F2CAG TCA CGA CGT TGT AAA ACG ACG GC62.028
R4*cCAG GAA ACA GCT ATG ACC ATG28
Acd31F15-31GAT CCT GGC TCA GAA TC56.73
Ver53F37-53TGG CGG CGT GGW TAA GA61.0D. Buckleyd
1492R1492-1510GGT TAC CTT GTT ACG ACT T30
  • a The positions of the target region are given using E. coli numbering system of the 16S rRNA gene. Primers F2 and R4* complement regions of the multiple cloning site of pCR2.1 or pCR4.0.

  • b Annealing temperatures optimized for the conditions used in this study are given for the forward primer of each primer pair.

  • c Primer R4* was modified from the primer in reference 28 by omission of five deoxynucleotides from the 5′ end.

  • d Personal communication.