TABLE 1.

PCR primers used in this study

PrimerPositionsaSequence (5′→3′)Annealing tempbReference or source
F2 CAG TCA CGA CGT TGT AAA ACG ACG GC 62.0 28
R4*c CAG GAA ACA GCT ATG ACC ATG 28
Acd31F15-31 GAT CCT GGC TCA GAA TC 56.7 3
Ver53F37-53 TGG CGG CGT GGW TAA GA 61.0D. Buckleyd
1492R1492-1510 GGT TAC CTT GTT ACG ACT T 30
  • a The positions of the target region are given using E. coli numbering system of the 16S rRNA gene. Primers F2 and R4* complement regions of the multiple cloning site of pCR2.1 or pCR4.0.

  • b Annealing temperatures optimized for the conditions used in this study are given for the forward primer of each primer pair.

  • c Primer R4* was modified from the primer in reference 28 by omission of five deoxynucleotides from the 5′ end.

  • d Personal communication.