TABLE 2.

Properties of PCR and RT-PCR and the different primer sets used for Q-PCR protocolsa

Target and primersReaction efficiencyCorrelation coefficient of regression lineMelting temp (°C)Detection limit of T. denitrificans-like nucleic acids ml−1b
BeforeAfterBeforeAfterBeforeAfterBeforeAfter
16S rRNA gene
    Com1.02 ± 0.020.99 ± 0.061.000 ± 0.0000.999 ± 0.00183.87 ± 0.1682.73 ± 0.36
    OST1.04 ± 0.150.97 ± 0.090.996 ± 0.0031.000 ± 0.00077.47 ± 0.1477.44 ± 0.151.4 × 1030.2
16S rRNA
    Com0.81 ± 0.070.88 ± 0.080.999 ± 0.0010.998 ± 0.00282.52 ± 0.2682.83 ± 0.33
    OST0.84 ± 0.070.89 ± 0.080.998 ± 0.0010.998 ± 0.00277.38 ± 0.1577.40 ± 0.177.4 × 10420
  • a Before, results without a preamplification step, using nucleic acids extracted from Bx enrichments; after, results with a preamplification step. Mean values of three or more determinations ± standard deviations are given.

  • b Values are numbers of copies.