TABLE 4.

MPN-PCR assay results

Borehole setOrganism(s) probedNo. of copies of 16S RNA gene/g of sediment (95% confidence limits)a
Before biostimulationAfter biostimulation
AGeobacter sp.5.38 × 103 (4.23 × 103-2.63 × 104)2.63 × 105 (2.19 × 105-8.50 × 105)
BGeobacter sp.5.38 × 103 (4.23 × 103-2.63 × 104)1.50 × 103 (1.06 × 103-7.38 × 103)
CGeobacter sp.2.25 × 103 (1.80 × 103-8.85 × 103)1.50 × 103 (1.06 × 103-7.38 × 103)
DGeobacter sp.6.50 × 102 (4.25 × 102-1.30 × 103)2.88 × 104 (2.38 × 104-1.50 × 105)
AAnaeromyxobacter sp.2.63 × 104 (2.10 × 104-1.03 × 105)1.16 × 103 (9.75 × 102-5.91 × 103)
BAnaeromyxobacter sp.2.63 × 104 (2.10 × 104-1.03 × 105)1.13 × 102 (1.00 × 102-5.63 × 102)
CAnaeromyxobacter sp.3.45 × 103 (2.25 × 103-1.46 × 104)5.38 × 103 (4.50 × 103-1.79 × 104)
DAnaeromyxobacter sp.4.25 × 104 (3.18 × 104-1.65 × 105)1.16 × 103 (9.75 × 102-5.91 × 103)
APaenibacillus-Brevibacillus spp.1.16 × 103 (9.75 × 102-5.91 × 103)1.43 × 102 (9.3 × 101-6.07 × 102)
BPaenibacillus-Brevibacillus spp.1.16 × 103 (9.75 × 102-5.91 × 103)1.00 × 100
CPaenibacillus-Brevibacillus spp.1.00 × 1006.98 × 103 (5.85 × 103-3.55 × 104)
DPaenibacillus-Brevibacillus spp.2.35 × 104 (1.65 × 104-7.85 × 104)1.88 × 102 (1.5 × 102-7.38 × 102)
  • a Quantitative PCR assay results obtained before and after biostimulation of contaminated sediment with glucose (comparing FB32 to nearby FB45 [borehole set A], FB32 to nearby FB46 [borehole set B], and FB33 to nearby FB47 [borehole set C]) and ethanol (comparing FB34 to nearby FB49 [borehole set D]) are shown.