TABLE 3.

Experimental set-up and results with the multivariate principal component analysis and the pET system

Expt no.IPTG addition (OD600)Temp (°C)Duration (h)Yield of active TrxRa
mg/litermg/liter/OD600
12.3371013.7 ± 6.43.7 ± 1.6
20.430481.5 ± 0.50.5 ± 0.1
31.8204013.6 ± 3.04.2 ± 1.2
41.83054.6 ± 3.21.6 ± 1.3
50.737243.3 ± 1.51.3 ± 0.4
63.037483.0 ± 0.021.5 ± 0.2
71.720247.8 ± 0.63.0 ± 0.2
81.82522.9 ± 1.31.1 ± 0.4
90.630165.3 ± 0.32.2 ± 0.8
100.037486.0 ± 5.11.8 ± 1.2
111.8202413.6 ± 1.64.6 ± 0.3
121.525168.6 ± 1.22.9 ± 0.5
  • a See Table 2, footnote a.