TABLE 1.

Strains and plasmids used in this study

Strain or plasmidCharacteristic(s)Reference or source
Strain
    E. coli EC1000RepA+ MC1000; Kmr; host for pOR128-based plasmids18
    L. acidophilus NCFMHuman intestinal isolate2
    L. acidophilus NCK1398NCFM integrant lacL::pTRK68525
    L. acidophilus NCK1392NCFM containing pTRK66925
    L. acidophilus NCK1678NCFM integrant ORF La57 (glutamate/GABA antiporter)::pTRK803This study
    L. acidophilus NCK1680NCFM integrant ORF La867 (transcriptional regulator)::pTRK804This study
    L. acidophilus NCK1682NCFM integrant ORF La995 (amino acid permease)::pTRK805This study
    L. acidophilus NCK1684NCFM integrant ORF La996 (ornithine decarboxylase)::pTRK806This study
Plasmid
    pTRK803576-bp internal region of ORF La57 cloned into the BgIII/Xbal sites of pOR128This study
    pTRK804593-bp internal region of ORF La867 cloned into the BgIII/Xbal sites of pOR128This study
    pTRK805604-bp internal region of ORF La995 cloned into the BgIII/Xbal sites of pOR128This study
    pTRK806624-bp internal region of ORF La996 cloned into the BgIII/Xbal sites of pOR128This study