TABLE 1.

Phylogenetic affiliations and numbers of bacterial 16S rRNA clones retrieved in libraries generated from density-resolved nucleic acidsa

Phylogenetic groupNo. of clonesCharacteristicb T-RFs (bp)
X3Ba (“heavy”)cX9Ba (“light”)c
δ-Proteobacteria
    Syntrophobacter spp.1469, 70, 167
    Smithella spp.5510
    Geobacter spp.16131
    WCH1B-27 group1163
Firmicutes
    Bacilli110146
    Clostridia550196, 207, 217, 273, 298, 513, 519
    Pelotomaculum spp.10151
    Desulfosporosinus spp.1142
    Selenomonas spp.1300
    Syntrophomonas spp.3302
Actinobacteria1136
Acidobacteria1?
Chlorobi1219
Bacteroidetes2140
Clone T78 group14516
Candidate division BRC-11494
Candidate division OP-1011165, 305
Unidentified1136
  • a Characteristic T-RFs for different clone groups are given.

  • b T-RFs were detected for a major group of clones within a lineage. T-RFs detected for more than one clone within a group are indicated in boldface.

  • c 13C enrichment of rRNA.