TABLE 3.

Tentative identification of dominant DGGE bands by sequencing the excised bands and BLAST analysisa

Band nameSequence size (bp)Accession no.Closest relative (accession no.)Alignment, % similarityScoreTaxonomic affiliation
Ca314 AY391554 Sinorhizobium sp. and Agrobacterium sp. (e.g., AJ300187 and L39882 )313/314, 99615α-Proteobacteria
Ma300 AY391555 Uncultured soil bacterium (AF507686 )290/292, 99563Unknown
Mb301 AY391556 Uncultured soil bacterium (AF507686 )289/292, 98555Unknown
Mc317 AY391557 Uncultured soil bacterium (AF507740 )312/317, 98589Unknown
Md315 AY391558 Uncultured acidobacterium (AY177759 )310/315, 98585 Acidobacteria
Me302 AY391559 Uncultured soil bacterium (e.g., AF423305 and AF423295 )294/294, 100583Unknown
NPKa313 AY391560 Uncultured bacterium (AB075101 )306/313, 97565Unknown
NPKb301 AY391561 Uncultured bacterium (AJ318200 )282/292, 96500Unknown
NPKc314 AY391562 Uncultured soil bacterium (AY326617 )304/314, 96543Unknown
NPKd309 AY391563 Massilia timonae (e.g., U54470 and AY157761 )306/309, 99589β-Proteobacteria
NPKe309 AY391564 β-Proteobacterium (AY278883 )306/309, 99589β-Proteobacteria
  • a Sequences were aligned with the closest relatives (highest score) in the GenBank database with BLAST. Bands correspond to those excised from the DGGE gel (Fig. 1). The percentage of similarity was calculated without taking gaps into account. The part of the total sequence used for alignment is indicated by the alignment data. Nucleotide sequences can be accessed by using the accession numbers at http://www.ncbi.nlm.nih.gov .