TABLE 2.

Phenotypes affected by heterologous expression of aiiA lactonases in B. thailandensis

SubstratePhenotype for straina:
DW503RU01RU02RU03RU04BTRJ1BTRJ3BTRJ6
C6-HSLDDDDNDDNDD
C8-HSLDDNDDNDNDDD
C10-HSLDDNDDNDDDD
Swarming motilityNANAMNAMMNANA
Twitching motilityNANAMNAMNANAA
β-HemolysisNANANANANAAAA
Generation timeNANAANANANANANA
α-Hydroxybutyric acidGNGNGNGNGGGG
l-ThreonineGNGNGNGNGGGG
l-AlanylglycinGNGNGNGNGGGG
d-XyloseGGGNGNGGGG
P-Hydroxy phenyl acetic acidGGNGGNGGGG
d-TrehaloseGNGGNGNGGGG
d-Glucosaminic acidGGGGNGGGG
α-Ketobutyric acidGGGGNGGGG
l-Aspartic acidGGNGGNGGGG
l-FucoseGGNGGGGGG
Acetic acidGGNGGGGGG
α-Ketoglutaric acidGGNGGGGGG
Glucose-1-phosphateGGNGGGGGG
Citric acidGGNGGGGGG
l-SerineGGNGGGGGG
GelatinGNGGNGGGGG
l-IsolcucineGNGNGNGGGGG
dl-CamitineGNGNGNGGNGGG
d-ArabinoseNGNGNGNGGNGGNG
d-ArabitolGGNGGGGGG
XylitolGGNGGGGGG
β-Hydroxybutyric acidGGNGGGGGG
Malonic acidGGNGGGGGG
l-PhenylalanineGGNGGGGGG
M9 minimal medium with d-arabinoseNGNGNGNGGNTNGNT
  • a D, detected; ND, not detected; M, colony morphology altered; NA, not affected; A, affected; G, growth; NG, no growth; NT, not tested. Strain genotypes are listed in Table 1.