TABLE 2.

Oligonucleotide primers used for plasmid construction

PrimerSequence (5′-3′)aF or RbDNA templateCassette
672GAA TAT GCA TGC GAT AAT GGC GCC TTT GCG GAA AGA GTT AGT AAG TTA ACA GFpT181, pRN8061, pSA0331pT181 replicon
671TGA ACG GGC CCA ATA AAA GCA ATC AAT GAA CCA AGRpT181, pRN8061, pSA0331pT181 replicon
P386TAC TTG GGG CCC TCG ATG ATT ACC AGA AGT TCT CRpI258pI258 replicon
P387TAG CAT GGC GCC GTT TTA TCT TCA TCA CTT GTA TTA ATCFpI258pI258 replicon
673GAT CAT CTC GAG CGG CCG CAT AGT TAA GCC AGC CCC GACFpUC19amp ColE1 ori
623GAT TAG CAT GCA GCG GCC GCC AGC TCA CTC AAA GGC GGRpUC19amp ColE1 ori
675CTC TGC GGG CCC ACC TAG GAA TTG AAT GAG ACA TGC TACFpE194ermC
674TCT CTT CTC GAG CGC CGC GGA AAA CTG GTT TAA GCC GACRpE194ermC
599GTT TAA GGG CCC ACC TAG GCA AAT ATG CTC TTA CGT GCFpRN6680tet(M)
600CTA TGA CTC GAG GCC GCG GAA ATA TTG AAG GCT AGT CAGRpR6680tet(M)
617GTT TAA GGG CCC ACC TAG GTA TTA TCA AGA TAA GAA AGFpC194cat194
618CTA TGA CTC GAG GCC GCG GCC TTC TTC AAC TAA CGG GGRpC194cat194
619GTT TAA GGG CCC ACC TAG GGG TTT CAA AAT CGG CTC CGFpAT21aphA-3
620CTA TGA CTC GAG GCC GCG GCG CTC GGG ACC CCT ATC TAG CRpAT21aphA-3
676GTT TAA GGG CCC ACC TAG GAT CGA ATC CCT TCG TGA GCGFTn554aad9
677TCT CTT CTC GAG GCC GCG GTA ATA AAC TAT CGA AGG AACRTn554aad9
583TAC GTT GCA TGC AGC TTA CTA TGC CAT TAT TFpI258PblaZ
584ATT CGT CTG CAG AAT AAA CCC TCC GAT ATT ACRpI258PblaZ
594TGC GAT GCA TGC GCA CTT ATT CAA GTG TAT TTFpI258Pcad-cadC
595AAT AAT GTC GAC CTG CAG GTT CAG ACA TTG ACC TTC ACRpI258Pcad-cadC
616TCG ATA GAA TTC GTT AAC TAA TTA ATA TCG GAG GGT TTA TTT TGFpI258blaZ
628AAC AGT GGC GCC TGT CAC TTT GCT TGA TAT ATG AGRpI258blaZ
589TCG ATA GAA TTC GTT AAC TAA TTA ATT TAA GAA GGA GAT ATA CAT ATG AGFpDB4gfpmut2
662AGC ATA GGC GCG CCT TAT TTG TAT AGT TCA TCC ATG CCARpDB4gfpmut2
601AAG ATT GAA TTC GTT AAC TAA TTA ATC ACC AAA AAG GAA TAG AGT ATG AAGFpRL189luxAB
612AAG TAT GGC GCG CCA AAA AGG CAA TCT AAT ATA GAA ATT GCCRpRL189luxAB
658TTA TCT GAA TTC AGG CGC GCC TAT TCT AAA TGC ATA ATA AAT ACT GFpI258TT
628AAC AGT GGC GCC TGT CAC TTT GCT TGA TAT ATG AGRpI258TT
655TTA ATC CGC GGT GTC ATT ATT TCFφ11φ11 EcoRI-K
656ATC TTC TCG AGT AAA AGG CTT CGG AAG TAGRφ11φ11 EcoRI-K
P805TTG ATG GGC CCG AAA GAT GTT GGT ATT GAT AAA GAA GFpRN6991SapI1-attS
P806TGG AAC CTA GGA GCT GGA AAT ATT CGT TAT TTA TGRpRN6991SapI1-attS
  • a Restriction sites are underlined. Bases complementary to the template are italicized. Bases in boldface represent stop codons.

  • b F, forward; R, reverse.