TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Source or reference
Strains
    E. coli
        DH5αHost for DNA cloningLab strain collection
        TOP10Host for DNA cloningInvitrogen Life Technologies
    S. aureus
        NCTC8325Propagating strain for typing phage 47Lab strain collection
        RN11Harbors pI258; lysogenic for φ11, φ12, and φ13Lab strain collection
        RN27RN450 lysogenic for 80α, φ1316
        RN450NCTC8325 cured of φ11, φ12, and φ1316
        RN13298325-4; lysogenic for φ11; Spr [Tn554(aad9)]Lab strain collection
        RN24248325 (pT181)Lab strain collection
        RN24428325-4 (pE194)Lab strain collection
        RN4220Restriction-defective derivative of RN45011
        RN42538325 (pRN8061)Lab strain collection
        RN4416RN27 (pSA0331)Lab strain collection
        RN58308325-4 (pC194)Lab strain collection
        RN6851RN6502 (pRN6680)Lab strain collection
        RN6734agr+; 8325-4 derivativeLab strain collection
        RN7206RN6734Δagr::tet(M)Lab strain collection
        RN7885JM109 (pRL189)Lab strain collection
        RN8972RN4220 (pRN6998)25
        RN9011RN4220 (pRN7023)25
Plasmids
    pUC19Apr; ColE1 compatibility group origin of replication 867; 2686 bp32
    pT181pT181wt; Tcr; Inc3; naturally occurring; ±22 copies/cell; 4,440 bp1, 17
    pRN8061pT181cop-623; Tcr; Inc3; ±400 copies/cell; 4,440 bp1, 17
    pSA0331pT181cop-634repC4; Tsr; Tcr; Inc3; ±145 copies/cell; 4,440 bp1, 17
    pI258Naturally occurring β-lactamase plasmid; 28 kb21
    pE194Emr (ermC); Inc11; naturally occurring; 3725 bp5
    pAT21Apr Kmr; pBR322Ω1.5-kb pJH1 ClaI (aphA-3)28
    pC194Cmr (cat194); Inc8; naturally occurring; 2,910 bp6
    pRN6680Apr Tcr; pBluescriptΩ2.9-kb pMVN6 Smal-HindII [tetA(M)]15
    pRN6998pE194tsΩpUC18-MCSΩSaPI1sek-int-attS; 8,319 bp25
    pRN7023pC194tsΩpUC19-MCSΩSaPI1int25
    pRL189luxABGift of Peter Wolk
    pBD4LITMUS28Ωgfpmut2-ermC; 4,784 bpGift of D. J. Bartels and E. P. Greenberg