TABLE 1.

Range of peak numbers and sizes in the ARISA profiles of six different environmental communities obtained with the three primer setsa

Environmental sampleValue for primer set
ITSF/ITSReubS-D-Bact-1522-b-S-20/L-D-Bact-132-a-A-181406F/23Sr
No. of peaks ± SDbRange of peak size (bp)Range of spacer size (bp)No. of peaks ± SDbRange of peak size (bp)Range of spacer size (bp)No. of peaks ± SDbRange of peak size (bp)Range of spacer size (bp)
Natural soil115 ± 6231-1,087109-96555 ± 3300-818114-6320 ± 0
Polluted soil41 ± 16245-698123-57642 ± 21307-737121-5510 ± 0
Urania brine36 ± 1218-99196-86912 ± 4285-72499-5383 ± 0593-759272-438
Ensiled corn33 ± 1294-1,030172-90829 ± 11318-1,056132-8702 ± 1570-1,088249-767
Goat milk34 ± 5229-1,463107-1,3415 ± 2293-603107-4174 ± 1444-746123-425
Certosa of Pavia marble8 ± 0228-894106-7723 ± 0300-879114-6931 ± 1579-608258-287
  • a The spacer sizes were calculated by subtracting the number of nucleotides belonging to 16S and 23S rRNA genes from the peak sizes.

  • b Data were calculated from three independent ARISA experiments.