TABLE 4.

Comparison of bacterial populations in GAM broth determined by real-time quantitative PCR, direct counting with DAPI staining, and the culture methoda

PrimerSpeciesStrainLog10 bacteria/ml as determined by:
qPCRbDAPICulture
g-CcocClostridium clostridiiformeYIT 6051T9.5 ± 0.19.5 ± 0.19.1 ± 0.1
Clostridium coccoidesYIT 6035T9.3 ± 0.29.4 ± 0.38.7 ± 0.1
Clostridium nexileYIT 6170T8.9 ± 0.18.7 ± 0.17.4 ± 0.3
Clostridium oroticumYIT 6037T8.6 ± 0.18.5 ± 0.18.3 ± 0.1
Clostridium sphenoidesYIT 6059T9.1 ± 0.29.2 ± 0.29.3 ± 0.1
Eubacterium rectaleYIT 6082T8.9 ± 0.18.9 ± 0.1<7.0
Ruminococcus gnavusYIT 6176T8.7 ± 0.28.7 ± 0.28.4 ± 0.1
Ruminococcus productusYIT 6141T9.5 ± 0.29.3 ± 0.29.1 ± 0.1
sg-CleptFaecalibacterium prausnitziiYIT 61748.9 ± 0.18.9 ± 0.2<7.0
Eubacterium siraeumYIT 10049T9.1 ± 0.18.9 ± 0.2<7.0
Ruminococcus bromiiYIT 6078T9.3 ± 0.19.2 ± 0.1<7.0
Ruminococcus callidusYIT 6175T8.5 ± 0.18.8 ± 0.2<7.0
Ruminococcus albusYIT 6083T8.3 ± 0.19.0 ± 0.1<7.0
Clostridium virideYIT 10050T8.0 ± 0.18.9 ± 0.1<7.0
Clostridium leptumYIT 6169T8.1 ± 0.29.1 ± 0.1<7.0
g-BfraBacteroides caccaeATCC 43185T9.4 ± 0.19.6 ± 0.18.8 ± 0.2
Bacteroides eggerthiiDSM 20697T9.0 ± 0.18.8 ± 0.19.4 ± 0.1
Bacteroides fragilisYIT 6158T9.4 ± 0.19.4 ± 0.28.3 ± 0.3
Bacteroides ovatusYIT 6161T9.1 ± 0.19.4 ± 0.28.5 ± 0.1
Bacteroides thetaiotaomicronYIT 6163T8.8 ± 0.19.0 ± 0.28.8 ± 0.2
Bacteroides uniformisYIT 6164T8.8 ± 0.18.8 ± 0.18.6 ± 0.1
Bacteroides vulgatusYIT 6159T8.7 ± 0.18.7 ± 0.18.6 ± 0.1
c-AtopoAtopobium parvulumYIT 10055T8.8 ± 0.28.7 ± 0.19.3 ± 0.1
Collinsella aerofaciensATCC 25986T9.3 ± 0.19.1 ± 0.18.9 ± 0.2
Collinsella intestinalisYIT 10051T9.2 ± 0.19.3 ± 0.110.0 ± 0.1
Collinsella stercorisYIT 10052T8.8 ± 0.29.0 ± 0.28.7 ± 0.2
Eggerthella lentaYIT 6077T8.1 ± 0.18.7 ± 0.28.2 ± 0.1
g-PrevoPrevotella corporisYIT 6132T9.0 ± 0.18.8 ± 0.28.7 ± 0.1
Prevotella denticolaYIT 61318.8 ± 0.18.9 ± 0.39.4 ± 0.1
Prevotella melaninogenicaYIT 6039T9.4 ± 0.19.2 ± 0.29.5 ± 0.1
Prevotella veroralisYIT 6126T8.8 ± 0.18.8 ± 0.18.9 ± 0.1
  • a A comparison of bifidobacterial species has been reported previously (17).

  • b PCR, real-time quantitative PCR.