TABLE 1.

In vitro luciferase regeneration profiles from 4-day culturesa

Time (h)Culture in medium without antibioticCulture in medium with rifampin
L. casei (log CFU · ml−1)Luciferase (log μRLU · CFU−1)L. casei (log CFU · ml−1)Luciferase (log μRLU · CFU−1)
0.008.4 ± 0.1NDb8.4 ± 0.1ND
0.258.4 ± 0.2ND8.2 ± 0.1ND
0.508.3 ± 0.2ND8.4 ± 0.1ND
1.508.4 ± 0.10.6 ± 0.28.4 ± 0.1ND
3.008.3 ± 0.21.9 ± 0.48.3 ± 0.1ND
  • a Regenerations were assessed by diluting (1/10) 4-day L. casei DN-114 001lux cultures in fresh GM medium. The growth of L. casei DN-114 001lux and luciferase activity were monitored until 3 h after the dilution. Regeneration experiments were performed in the absence of antibiotic or with the addition of rifampin.

  • b ND, no detectable luciferase activity.