TABLE 1.

Concentration, δ15N, δ18O, and site preference of N2O produced during NH2OH oxidation by concentrated cell suspensions of N. europaea, N. multiformis, and M. trichosporium

Organism and replicateaTime elapsed (min)[N2O] (μM)δ15N-N2O (‰)δ18O-N2O (‰)Site preference (‰)
Nitrosomonas europaea
    A30410.8−5.535.834.5
    A39010.5−5.535.534.6
    A44210.9−4.837.031.1
    B13512.63.840.833.1
    B31515.24.642.531.9
    B39516.65.142.332.1
    C2896.80.638.433.7
    C4296.8−1.638.337.5
    C5076.70.938.533.1
        Avg−0.338.833.5
        SD4.92.91.2
Nitrosospira multiformis
    A2607.5−3.635.633.1
    A3698.8−3.935.532.1
    A4229.3−3.536.134.2
    B2157.51.340.031.4
    B2708.50.939.730.7
    B3158.80.939.933.6
    C4118.61.738.032.7
    C4909.91.641.231.8
    C57310.11.641.832.8
        Avg−0.338.632.5
        SD2.92.50.6
Methylosinus trichosporium
    A1655.71.435.137.3
    A1855.71.435.435.0
    A2056.31.335.637.5
    B9512.75.239.234.9
    B15519.55.040.433.3
    B19523.34.741.135.1
    B29030.84.242.635.0
    B33030.94.241.835.0
    B450383.945.734.4
        Avg3.439.735.6
        SD1.94.01.4
  • a Replicates (A, B, or C) represent experiments conducted on separate days with different cultures sampled over time.