TABLE 2.

Selected plasmids generated in this studya

Use and plasmidDescriptionb
Complementation and gene introduction
    pVSV104Knr, lacZα-(SphI, AvrII, HpaI, SalI, NcoI, KpnI, SacI)
    pVSV105Cmr, lacZα-(SphI, SalI/HincII, XbaI, SmaI/XmaI, KpnI, SacI)
    pVSV106Tcr, lacZα-(ApaI, XhoI, SalI/HincII, SmaI/XmaI, XbaI)
    pVSV107Tpr, lacZα-(KpnI, ApaI, XhoI, SalI, SpeI, XbaI, NotI, SacI)
Copy number variant
    pVSV104HpVSV104 with single-base-pair change in origin and increased copy number
Strain tagging
    pVSV102Knr, gfp
    pVSV103Knr, lacZ
    pVSV208Cmr, rfp
Gene expression analyses
    pVSV33Knr, transcriptional terminators-(SphI, AvrII, KpnI, XbaI, SalI, StuI)-promoterless Cmr and gfp
    pVSV209Knr, rfp, transcriptional terminators-(AvrII, SalI, StuI)-promoterless Cmr and gfp
    pVSV210Knr, rfp, Plux-cat-gfp
  • a Other plasmids are described in Materials and Methods.

  • b All plasmids in this table contain the RP4 oriT and the pES213 and R6Kγ replication origins. Knr, kanamycin resistance; Cmr, chloramphenicol resistance; Tcr, tetracycline resistance; Tpr, trimethoprim resistance. Parentheses bracket unique restriction sites present in the multiple cloning site.