TABLE 3.

Identification of acid streamer isolates based on analysis of 16S rRNA gene sequences

IsolateGenBank accession no.Physiological classaClosest cultivated relative (GenBank accession no.)% Identity
Mynydd Parys streamer isolates
    PK11NSbIron-oxidizing autotrophAcidithiobacillus ferrooxidans NO-37 (AF376020)99.9
    KP1AY765991Iron-oxidizing heterotroph“Ferrimicrobium acidiphilum” (AF251436)93.1
    KP3AY765992HeterotrophAcidobacterium capsulatum (D26171)91.8
    PK35AY765993HeterotrophWheal Jane isolate WJ7 (AY096034)99.7
    PK40AY765994HeterotrophAcidiphilium sp. strain C-1 (D30769)99.7
    PK44AY455806HeterotrophThiomonas sp. strain NO115 (AY455807)99.5
    PK46AY765995HeterotrophAcidiphilium cryptum ATCC 33463T (D30773)99.9
    PK48AY765996HeterotrophAcidiphilium acidophilum ATCC 27807T (D86511)97.5
    PK51AY765997HeterotrophWheal Jane isolate WJ2 (AY096032)100
    M21AY765998HeterotrophAcidocella sp. strain NO-12 (AF376021)99.1
Trefriw Spa streamer isolates
    CCW10NSIron-oxidizing autotrophAcidithiobacillus ferrooxidans NO-37 (AF376020)99.7
    CCW68NSIron-oxidizing autotrophAcidithiobacillus ferrooxidans ATCC 23270T (AF465604)99.5
    CS11AY765999Iron-oxidizing heterotroph“Ferrimicrobium acidiphilum” (AF251436)93.1
    CCP3AY766000HeterotrophAcidiphilium sp. strain NO-17 (AF376026)99.5
    CCW30AY766001HeterotrophAcidocella sp. strain NO-12 (AF376021)99.4
  • a Physiological class refers to the phenotype initially used to discriminate isolates.

  • b NS, not submitted to GenBank.