TABLE 2.

TaqMan probes and primers used to quantify Dehalococcoides spp. RDase genes and total bacterial 16S rRNA genes

Primer/probeSequenceTarget geneReference(s) or source
Dhc1200F5′-CTGGAGCTAATCCCCAAAGCT Dehalococcoides sp. 16S rDNA 15
Dhc1271R5′-CAACTTCATGCAGGCGGG Dehalococcoides sp. 16S rDNA 15
Dhc1240Probe5′-FAM-TCCTCAGTTCGGATTGCAGGCTGAA-TAMRA Dehalococcoides sp. 16S rDNA 15
Bvc925F5′-AAAAGCACTTGGCTATCAAGGAC bvcA geneThis study
Bvc1017R5′-CCAAAAGCACCACCAGGTC bvcA geneThis study
Bvc977Probe5′-FAM-TGGTGGCGACGTGGCTATGTGG-TAMRA bvcA geneThis study
Vcr1022F5′-CGGGCGGATGCACTATTTT vcrA geneThis study
Vcr1093R5′-GAATAGTCCGTGCCCTTCCTC vcrA geneThis study
Vcr1042Probe5′-FAM-CGCAGTAACTCAACCATTTCCTGGTAGTGG-TAMRA vcrA geneThis study
TceA1270F5′-ATCCAGATTATGACCCTGGTGAA tceA gene 3, 22
TceA1336R5′-GCGGCATATATTAGGGCATCTT tceA gene 3, 22
TceA1294Probe5′-FAM-TGGGCTATGGCGACCGCAGG-TAMRA tceA gene 3, 22
Bac1055YFa5′-ATGGYTGTCGTCAGCT Bacteria This study; 11
Bac1392R5′-ACGGGCGGTGTGTAC Bacteria 25
Bac1115Probe5′-FAM-CAACGAGCGCAACCC-TAMRA Bacteria 14, 25
  • a The C in the original reference was modified to a Y (which equals C/T).