TABLE 2.

TaqMan probes and primers used to quantify Dehalococcoides spp. RDase genes and total bacterial 16S rRNA genes

Primer/probeSequenceTarget geneReference(s) or source
Dhc1200F5′-CTGGAGCTAATCCCCAAAGCTDehalococcoides sp. 16S rDNA15
Dhc1271R5′-CAACTTCATGCAGGCGGGDehalococcoides sp. 16S rDNA15
Dhc1240Probe5′-FAM-TCCTCAGTTCGGATTGCAGGCTGAA-TAMRADehalococcoides sp. 16S rDNA15
Bvc925F5′-AAAAGCACTTGGCTATCAAGGACbvcA geneThis study
Bvc1017R5′-CCAAAAGCACCACCAGGTCbvcA geneThis study
Bvc977Probe5′-FAM-TGGTGGCGACGTGGCTATGTGG-TAMRAbvcA geneThis study
Vcr1022F5′-CGGGCGGATGCACTATTTTvcrA geneThis study
Vcr1093R5′-GAATAGTCCGTGCCCTTCCTCvcrA geneThis study
Vcr1042Probe5′-FAM-CGCAGTAACTCAACCATTTCCTGGTAGTGG-TAMRAvcrA geneThis study
TceA1270F5′-ATCCAGATTATGACCCTGGTGAAtceA gene3, 22
TceA1336R5′-GCGGCATATATTAGGGCATCTTtceA gene3, 22
TceA1294Probe5′-FAM-TGGGCTATGGCGACCGCAGG-TAMRAtceA gene3, 22
Bac1055YFa5′-ATGGYTGTCGTCAGCTBacteriaThis study; 11
Bac1392R5′-ACGGGCGGTGTGTACBacteria25
Bac1115Probe5′-FAM-CAACGAGCGCAACCC-TAMRABacteria14, 25
  • a The C in the original reference was modified to a Y (which equals C/T).